@tin14miceactive 5 days, 21 hours ago
Progeny were dissected at 6.5 or 7.5 dpc in ice cold medium. Embryos were staged according to Downs and Davies (Downs and Davies, 1993). For time-lapse imaging, mT/mG embryos were examined for EGFP expression, and EGFP-positive embryos were then staged and oriented on glass-bottom culture dishes with their posterior sides facing down. They were […] View
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